8 詳細
2005年4月〜 東京大学大学院薬学系研究科 薬化学教室 助教 > 投稿論文(共同研究)
Difference in intracellular temperature rise between matured and precursor brown adipocytes in response to uncoupler and β-adrenergic agonist stimuli
Toshikazu Tsuji, Kumiko Ikado, Hideki Koizumi, Seiichi Uchiyama, Kazuaki KajimotoSci. Rep., 2017, 7, 12889
■ Abstract
Brown adipocytes function to maintain body temperature by heat production. However, direct measurement of heat production at a single cell level remains difficult. Here we developed a method to measure the temperature within primary cultured brown adipocytes using a cationic fluorescent polymeric thermometer. Placement of the thermometer within a matured brown adipocyte and a precursor cell enabled the detection of heat production following uncoupler treatment. The increase in the intracellular temperature due to stimulation with a mitochondrial uncoupler was higher in matured brown adipocytes than in precursor cells. Stimulation with a β-adrenergic receptor (β-AR) agonist, norepinephrine, raised the intracellular temperature of matured brown adipocytes to a level comparable to that observed after stimulation with a β3-AR-specific agonist, CL316.243. In contrast, neither β-AR agonist induced an intracellular temperature increase in precursor cells. Further, pretreatment of brown adipocytes with a β3-AR antagonist inhibited the norepinephrine-stimulated elevation of temperature. These results demonstrate that our novel method successfully determined the difference in intracellular temperature increase between matured brown adipocytes and precursor cells in response to stimulation by an uncoupler and β-AR agonists.
■ 内容
2015年に報告したレシオ型蛍光性温度センサーを利用して褐色脂肪細胞の温度計測を行いました．褐色脂肪細胞はミトコンドリアの含有量も多く，ほ乳類におけるエネルギー消費器官として注目を集めています．自分の開発したレシオ型蛍光性温度センサーを使って，褐色脂肪細胞の温度を測る，という試みは平行していくつかの研究グループが進めており，Sci. Rep., 2017, 7, 12978やFeseb. J., 2017, 31(suppl), 886.14（査読付き論文は未発表）でも報告が相次いでいます．