3 詳細
Fluorescent oxidation products derived from DBD-thiocarbamoyl amino acid in the modified Edman sequencing analysis
Y. Huang, T. Santa, S. Uchiyama and K. Imai
Biomed. Chromatogr., 2000, 14, 133-136.
■ Abstract
The fluorescent product obtained by the oxidation of 7-N,N-dimethylaminosulfonyl-4-(2, 1,3-benzoxadiazolyl) (DBD)-thiocarbamoyl (TC)-proline with NaNO2/H+ in the modified Edman sequencing procedure was identified as the corresponding thiazolyl compound, N-[(8-dimethylaminosulfonyl)thiazolo [5,4,e]benzo[2,1,3]oxadiazol-5-yl]-L-proline, formed by the attack of the sulfur atom of the thiocarbamoyl group on the benzofurazan skeleton. The reaction mechanism for the formation of the fluorescent compound from DBD-TC primary and secondary amines is also discussed.
■ ひとこと
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