19 詳細
2005年4月〜 東京大学大学院薬学系研究科 薬化学教室 助教 > 投稿論文
Luminescent molecular thermometers for the ratiometric sensing of intracellular temperature
Seiichi Uchiyama and Chie GotaRev. Anal. Chem., 2017, 36(1), DOI:10.1515/revac-2016-0021
■ Abstract
Recently, numerous luminescent molecular thermometers that exhibit temperature-dependent emission properties have been developed to measure the temperatures of tiny spaces. Intracellular temperature is the most interesting and exciting applications of luminescent molecular thermometers because this temperature is assumed to be correlated with all cell events, such as cell division, gene expression, enzyme reaction, metabolism, and pathogenesis. Among the various types of temperature- dependent emission parameters of luminescent molecular thermometers, the emission intensity ratio at two different wavelengths is suitable for accurate and accessible intracellular temperature measurements. In this review article, luminescent molecular thermometers that exhibit a temperature-dependent emission intensity ratio in living cells are summarized, and current progress in intracellular thermometry is outlined.
■ 内容
細胞内温度計測用のレシオ型蛍光性温度センサーを網羅的にまとめた総説です．
■ ひとこと
OMM(Open My Mind)政策を取り入れてすぐに，この総説の執筆依頼が届きました．Pure Appl. Chem.を発行している出版社ということもあって，快諾しました．この総説のテーマである「レシオ型蛍光プローブを利用した細胞内の温度測定」は，先方が最初に要望してきたたものです．2016年5月頃までの論文をまとめてあります．